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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Ala<sup>5</sup>-Galanin (2-11)
    Bioassay Tested
    Ala5-Galanin (2-11)
    A potent agonist selective for the GALR2 Galanin receptors
    • Product Code: GPG-300
    • Short Description: A potent agonist selective for the GALR2 Galanin receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • MW: 1120 Da
  • AM 404
    Bioassay Tested
    AM 404
    An Activator of TRPV1 Channels and a Ligand of CB1 Cannabinoid Receptors
    • Product Code: A-190
    • Short Description: An Activator of TRPV1 Channels and a Ligand of CB1 Cannabinoid Receptors
    • CAS No.: 183718-77-6, 198022-70-7
    • MW: 395.6
  • AMG 9810
    Bioassay Tested
    AMG 9810
    A Blocker of TRPV1 Channels
    • Product Code: A-180
    • Short Description: A Blocker of TRPV1 Channels
    • CAS No.: 545395-94-6
    • MW: 337.4
  • AMG-9090
    Bioassay Tested
    AMG-9090
    A Blocker of TRPA1, TRPV1 and TRPM8 Channels
    • Product Code: A-300
    • Short Description: A Blocker of TRPA1, TRPV1 and TRPM8 Channels
    • CAS No.: 118215-82-0
    • MW: 395.12
  • AMG2850
    Bioassay Tested
    AMG2850
    A Potent and Selective Blocker of TRPM8 Channels
    • Product Code: A-340
    • Short Description: A Potent and Selective Blocker of TRPM8 Channels
    • CAS No.: 1470018-52-0
    • MW: 417.35
  • AMTB hydrochloride
    Bioassay Tested
    AMTB hydrochloride
    A Blocker of TRPM8 Channels
    • Product Code: A-305
    • Short Description: A Blocker of TRPM8 Channels
    • CAS No.: 926023-82-7
    • MW: 431
  • Anti-Aquaporin 11 (extracellular) Antibody
    Anti-Aquaporin 11 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Product Code: AQP-011
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: AQP11
  • Anti-ASIC1 Antibody
    KO Validate'
    Anti-ASIC1 Antibody
    A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Product Code: ASC-014
    • Short Description: A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • Anti-ASIC2a Antibody
    KO Validate'
    Anti-ASIC2a Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Product Code: ASC-012
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC2a-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-ASIC2a-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
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