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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-027
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Anti-Na<sub>V</sub>1.7 (SCN9A) Antibody
    KO Validate'
    Anti-NaV1.7 (SCN9A) Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-008
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Anti-Na<sub>V</sub>1.7 (SCN9A)-ATTO Fluor-633 Antibody
    Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ASC-008-FR
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Anti-Na<sub>V</sub>1.8 (SCN10A) (extracellular) Antibody
    Anti-NaV1.8 (SCN10A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-028
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-Na<sub>V</sub>1.8 (SCN10A) Antibody
    KO Validate'
    Anti-NaV1.8 (SCN10A) Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-016
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-Na<sub>V</sub>1.8 (SCN10A)-ATTO Fluor-594 Antibody
    Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-016-AR
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-Neurokinin 2 Receptor (NK2R) Antibody
    Anti-Neurokinin 2 Receptor (NK2R) Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 2 Receptor
    • Product Code: ATR-002
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: NK2 receptor, Neurokinin A receptor, Tachykinin receptor 2, TACR2, Substance-K receptor, SKR
  • Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody
    Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 1 (extracellular)
    • Product Code: ANR-073
    • Short Description: A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022
  • Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody
    Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)
    • Product Code: ANR-074
    • Short Description: A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: NPFFR2, G-Protein Coupled Receptor 74, GPR74
  • Anti-NMDAR1 (GluN1) (extracellular) Antibody
    Anti-NMDAR1 (GluN1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Product Code: AGC-001
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
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