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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody
    Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AOR-015-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor
  • Anti-P2X1 Receptor (extracellular) Antibody
    Anti-P2X1 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Product Code: APR-022
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
  • Anti-P2X1 Receptor (extracellular)-APC Antibody
    Anti-P2X1 Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APR-022-APC
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2RX1, P2X purinoceptor 1, ATP Receptor
  • Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-022-AG
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
  • Anti-P2X1 Receptor (extracellular)-FITC Antibody
    Anti-P2X1 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-022-F
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2RX1, P2X purinoceptor 1
  • Anti-P2X1 Receptor Antibody
    KO Validate'
    Anti-P2X1 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Product Code: APR-001
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
  • Anti-P2X2 Receptor (extracellular) Antibody
    Anti-P2X2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X2 Receptor
    • Product Code: APR-025
    • Short Description: A Rabbit Polyclonal Antibody to P2X2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: P2X purinoceptor 2, P2RX2
  • Anti-P2X2 Receptor Antibody
    KO Validate'
    Anti-P2X2 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2X2 Receptor
    • Product Code: APR-003
    • Short Description: A Rabbit Polyclonal Antibody to P2X2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: P2X purinoceptor 2, P2RX2
  • Anti-P2X2 Receptor-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-P2X2 Receptor-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to P2X2 Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APR-003-AR
    • Short Description: A Rabbit Polyclonal Antibody to P2X2 Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: P2X purinoceptor 2, P2RX2
  • Anti-P2X3 Receptor (extracellular) Antibody
    KO Validate'
    Anti-P2X3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X3 Receptor
    • Product Code: APR-026
    • Short Description: A Rabbit Polyclonal Antibody to P2X3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX3, P2X purinoceptor 3
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