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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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771 Results
  • GTx1-15
    A blocker of voltage-gated Na+ and T-Type Ca2+ channels
    • Product Code: STT-300
    • Short Description: A blocker of voltage-gated Na+ and T-Type Ca2+ channels
    • Applications: Electrophysiology
    • MW: 4073.8 Da
  • Guinea pig Anti-ASIC1 Antibody
    A Guinea Pig Polyclonal Antibody to ASIC1 Channel
    • Product Code: ASC-014-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to ASIC1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • Guinea pig Anti-ASIC3 Antibody
    A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Product Code: ASC-018-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
  • Guinea pig Anti-HCN1 Antibody
    A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Guinea pig Anti-KCNK2 (TREK-1) Antibody
    A Guinea Pig Polyclonal Antibody to KCNK2 Channel
    • Product Code: APC-047-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNK2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
  • Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody
    A Guinea Pig Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor
    • Product Code: AMR-061-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor
  • Guinea pig Anti-Na<sub>V</sub>1.7 (SCN9A) Antibody
    A Guinea Pig Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-008-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Guinea pig Anti-Na<sub>V</sub>1.8 (SCN10A) Antibody
    A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Product Code: AGC-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
  • Guinea Pig Anti-P2X3 Antibody
    A Guinea Pig Polyclonal Antibody to P2X3 Receptor
    • Product Code: APR-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to P2X3 Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: P2RX3, P2X purinoceptor 3, ATP Receptor
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