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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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970 Results
  • Anti-CHRM1 (443-458) Antibody
    Anti-CHRM1 (443-458) Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Product Code: AMR-010
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
  • Anti-CHRM1 Antibody
    KO Validate'
    Anti-CHRM1 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Product Code: AMR-001
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
  • Anti-CHRM2 Antibody
    KO Validate'
    Anti-CHRM2 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M2
    • Product Code: AMR-002
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Muscarinic acetylcholine receptor M2, Cholinergic receptor muscarinic 2, mAChR M2
  • Anti-CHRM3 Antibody
    KO Validate'
    Anti-CHRM3 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M3
    • Product Code: AMR-006
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 3, Muscarinic acetylcholine receptor M3, M3 mAChR
  • Anti-CNGA1 Antibody
    Anti-CNGA1 Antibody
    A Rabbit Polyclonal Antibody to CNGA1 Channel
    • Product Code: APC-071
    • Short Description: A Rabbit Polyclonal Antibody to CNGA1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot
    • Target: Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49
  • Anti-CNGA2 Antibody
    Anti-CNGA2 Antibody
    A Rabbit Polyclonal Antibody to CNGA2 Channel
    • Product Code: APC-045
    • Short Description: A Rabbit Polyclonal Antibody to CNGA2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Cyclic nucleotide-gated cation channel 2, CNG channel alpha-2, CNG2, CNCG2, Olfactory cyclic nucleotide gated channel 1, OCNC1
  • Anti-CNGA3 Antibody
    Anti-CNGA3 Antibody
    A Rabbit Polyclonal Antibody to CNGA3 Channel
    • Product Code: APC-060
    • Short Description: A Rabbit Polyclonal Antibody to CNGA3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2
  • Anti-CNGA4 Antibody
    Anti-CNGA4 Antibody
    A Rabbit Polyclonal Antibody to CNGA4 Channel
    • Product Code: APC-074
    • Short Description: A Rabbit Polyclonal Antibody to CNGA4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cyclic nucleotide-gated cation channel alpha-4, CNG4, Cyclic nucleotide-gated olfactory channel subunit OCNC2, OCNCb
  • Anti-CNIH2/3 (extracellular) Antibody
    Anti-CNIH2/3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CNIH2 and CNIH3
    • Product Code: AGC-047
    • Short Description: A Rabbit Polyclonal Antibody to CNIH2 and CNIH3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3
  • Anti-CPLX1/2 Antibody
    Anti-CPLX1/2 Antibody
    A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2
    • Product Code: ANR-011
    • Short Description: A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1
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