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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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970 Results
  • Anti-GRIP1 Antibody
    Anti-GRIP1 Antibody
    A Rabbit Polyclonal Antibody to GRIP1
    • Product Code: APZ-015
    • Short Description: A Rabbit Polyclonal Antibody to GRIP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Glutamate receptor interacting protein 1
  • Anti-GSG1L Antibody
    Anti-GSG1L Antibody
    A Rabbit Polyclonal Antibody to GSG1L
    • Product Code: AGC-048
    • Short Description: A Rabbit Polyclonal Antibody to GSG1L
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Germ cell-specific gene 1-like protein, GSG1-like protein
  • Anti-HCN1 Antibody
    KO Validate'
    Anti-HCN1 Antibody
    A Rabbit Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056
    • Short Description: A Rabbit Polyclonal Antibody to HCN1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Anti-HCN2 Antibody
    KO Validate'
    Anti-HCN2 Antibody
    A Rabbit Polyclonal Antibody to HCN2 Channel
    • Product Code: APC-030
    • Short Description: A Rabbit Polyclonal Antibody to HCN2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • Anti-HCN2-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-HCN2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APC-030-AR
    • Short Description: A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • Anti-HCN3 (extracellular) Antibody
    Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN3 Antibody
    Anti-HCN3 Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-057
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-Histamine H<sub>1 </sub> Receptor/HRH1 (extracellular) Antibody
    Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Product Code: AHR-006
    • Short Description: A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Histamine Receptor H1, H1R
  • Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AHR-006-F
    • Short Description: A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Histamine Receptor H1, H1R
  • Anti-Homer1 Antibody
    Anti-Homer1 Antibody
    A Rabbit Polyclonal Antibody to Homer1
    • Product Code: APZ-026
    • Short Description: A Rabbit Polyclonal Antibody to Homer1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47
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