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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-Homer2 Antibody
    Anti-Homer2 Antibody
    A Rabbit Polyclonal Antibody to Homer2
    • Product Code: APZ-027
    • Short Description: A Rabbit Polyclonal Antibody to Homer2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 2, Cupidin, Vesl-2
  • Anti-Homer3 Antibody
    Anti-Homer3 Antibody
    A Rabbit Polyclonal Antibody to Homer3
    • Product Code: APZ-028
    • Short Description: A Rabbit Polyclonal Antibody to Homer3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3
  • Anti-Human Adenosine A<sub>2A</sub> Receptor (extracellular) Antibody
    KO Validate'
    Anti-Human Adenosine A2A Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-007
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Adenosine receptor A2A, A2AR, ADORA2A
  • Anti-Human Adenosine A<sub>2A</sub> Receptor (extracellular)-FITC Antibody
    Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Adenosine receptor A2A, A2AR, ADORA2A
  • Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody
    Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Product Code: ACR-003
    • Short Description: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: CB2, CNR2
  • Anti-Human Ca<sub>V</sub>1.2 (CACNA1C) Antibody
    Anti-Human CaV1.2 (CACNA1C) Antibody
    A Rabbit Polyclonal Antibody to Human CaV1.2 (CACNA1C) Channel
    • Product Code: ACC-022
    • Short Description: A Rabbit Polyclonal Antibody to Human CaV1.2 (CACNA1C) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Human α1C, Voltage-dependent L-type calcium channel subunit alpha-1C.
  • Anti-Human GPR55 (extracellular) Antibody
    Anti-Human GPR55 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR55
    • Product Code: ACR-061
    • Short Description: A Rabbit Polyclonal Antibody to GPR55
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: G-protein coupled receptor 55
  • Anti-Human Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Product Code: ASC-029
    • Short Description: A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1
  • Anti-Human TRPV1 (extracellular) Antibody
    Anti-Human TRPV1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Product Code: ACC-334
    • Short Description: A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV1 (extracellular)-APC Antibody
    Anti-Human TRPV1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACC-334-APC
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
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