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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-Human TRPV1 (extracellular)-FITC Antibody
    Anti-Human TRPV1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-334-F
    • Short Description: A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV2
    • Product Code: ACC-139
    • Short Description: A Rabbit Polyclonal Antibody to TRPV2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B
  • Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-139-F
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2
  • Anti-KChIP1 (KCNIP1) Antibody
    Anti-KChIP1 (KCNIP1) Antibody
    A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Product Code: APC-141
    • Short Description: A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1
  • Anti-KChIP2 Antibody
    Anti-KChIP2 Antibody
    A Rabbit Polyclonal Antibody to KChIP2 Subunit
    • Product Code: APC-142
    • Short Description: A Rabbit Polyclonal Antibody to KChIP2 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit
  • Anti-KChIP3 (CSEN, DREAM) Antibody
    Anti-KChIP3 (CSEN, DREAM) Antibody
    A Rabbit Polyclonal Antibody to KChIP3 (CSEN, DREAM) Subunit
    • Product Code: APC-143
    • Short Description: A Rabbit Polyclonal Antibody to KChIP3 (CSEN, DREAM) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator
  • Anti-KCNC3 (K<sub>V</sub>3.3) Antibody
    KO Validate'
    Anti-KCNC3 (KV3.3) Antibody
    A Rabbit Polyclonal Antibody to KCNC3 (KV3.3) Channel
    • Product Code: APC-102
    • Short Description: A Rabbit Polyclonal Antibody to KCNC3 (KV3.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13
  • Anti-KCNG1 (K<sub>V</sub>6.1) (extracellular) Antibody
    Anti-KCNG1 (KV6.1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNG1 (KV6.1) Channel
    • Product Code: APC-135
    • Short Description: A Rabbit Polyclonal Antibody to KCNG1 (KV6.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily G member 1
  • Anti-KCNH1 (EAG-1) Antibody
    Anti-KCNH1 (EAG-1) Antibody
    A Rabbit Polyclonal Antibody to KV10.1 Channel
    • Product Code: APC-104
    • Short Description: A Rabbit Polyclonal Antibody to KV10.1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: KV10.1, Ether-a-go-go potassium channel 1, hEAG1
  • Anti-KCNH5 (EAG-2) Antibody
    KO Validate'
    Anti-KCNH5 (EAG-2) Antibody
    A Rabbit Polyclonal Antibody to KV10.2 Channel
    • Product Code: APC-053
    • Short Description: A Rabbit Polyclonal Antibody to KV10.2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KV10.2, Ether-a-go-go potassium channel 2, hEAG2
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