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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-NCS1 Antibody
    Anti-NCS1 Antibody
    A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1
    • Product Code: ACS-001
    • Short Description: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • Anti-NCS1-ATTO Fluor-594 Antibody
    Anti-NCS1-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACS-001-AR
    • Short Description: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • Anti-NEGR1 (extracellular) Antibody
    Anti-NEGR1 (extracellular) Antibody
    A Rabbit polyclonal antibody to NEGR1 (extracellular).
    • Product Code: ANR-058
    • Short Description: A Rabbit polyclonal antibody to NEGR1 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Neuronal Growth Regulator 1, IgLON Family Member 4, IGLON4 
  • Anti-NETO1 (extracellular) Antibody
    Anti-NETO1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NETO1
    • Product Code: AGC-045
    • Short Description: A Rabbit Polyclonal Antibody to NETO1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1
  • Anti-NETO2 (extracellular) Antibody
    Anti-NETO2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NETO2
    • Product Code: AGC-046
    • Short Description: A Rabbit Polyclonal Antibody to NETO2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, BTCL2
  • Anti-Neurexin 1α (extracellular) Antibody
    Anti-Neurexin 1α (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neurexin 1α
    • Product Code: ANR-031
    • Short Description: A Rabbit Polyclonal Antibody to Neurexin 1α
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Nrxn1, Neurexin I-alpha
  • Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody
    Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Neurexin 1α Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANR-031-AG
    • Short Description: A Rabbit Polyclonal Antibody to Neurexin 1α Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Nrxn1, Neurexin I-alpha
  • Anti-Neurexin 3α (extracellular) Antibody
    Anti-Neurexin 3α (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neurexin 3α
    • Product Code: ANR-033
    • Short Description: A Rabbit Polyclonal Antibody to Neurexin 3α
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nrxn3, NRX3A, Neurexin III-alpha
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor
    • Product Code: ATR-001
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunohistochemistry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-APC Antibody
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye Allophycocyanin (APC).
    • Product Code: ATR-001-APC
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye Allophycocyanin (APC).
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
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