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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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970 Results
  • Anti-Oxytocin Receptor Antibody
    KO Validate'
    Anti-Oxytocin Receptor Antibody
    A Rabbit Polyclonal Antibody to Oxytocin Receptor
    • Product Code: AVR-013
    • Short Description: A Rabbit Polyclonal Antibody to Oxytocin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: OXTR, OT-R, OTR1
  • Anti-PACAP Receptor 1 (PAC1) Antibody
    KO Validate'
    Anti-PACAP Receptor 1 (PAC1) Antibody
    A Rabbit Polyclonal Antibody to PACAP Type I Receptor
    • Product Code: AVR-003
    • Short Description: A Rabbit Polyclonal Antibody to PACAP Type I Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1
  • Anti-pan ASIC (extracellular) Antibody
    Anti-pan ASIC (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Product Code: ASC-031
    • Short Description: A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4
  • Anti-Pan Na<sub>V</sub> Antibody
    Anti-Pan NaV Antibody
    A Rabbit Polyclonal Antibody to Pan NaV Channel
    • Product Code: ASC-003
    • Short Description: A Rabbit Polyclonal Antibody to Pan NaV Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Pan sodium channel, SP19
  • Anti-PICK1 Antibody
    Anti-PICK1 Antibody
    A Rabbit Polyclonal Antibody to PICK1
    • Product Code: APZ-014
    • Short Description: A Rabbit Polyclonal Antibody to PICK1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein
  • Anti-PMCA2 Antibody
    Anti-PMCA2 Antibody
    A Rabbit Polyclonal Antibody to PMCA2 ATPase
    • Product Code: ACP-002
    • Short Description: A Rabbit Polyclonal Antibody to PMCA2 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Plasma membrane Ca2+ ATPase 2, ATP2B2
  • Anti-proBDNF Antibody
    Anti-proBDNF Antibody
    A Rabbit Polyclonal Antibody to proBDNF
    • Product Code: ANT-006
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-proBDNF-ATTO Fluor-488 Antibody
    Anti-proBDNF-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-proNT-3 Antibody
    Anti-proNT-3 Antibody
    A Rabbit Polyclonal Antibody to proNT-3
    • Product Code: ANT-012
    • Short Description: A Rabbit Polyclonal Antibody to proNT-3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: pro-Neurotrophin 3, proNTF3
  • Anti-PSD-93 Antibody
    KO Validate'
    Anti-PSD-93 Antibody
    A Rabbit Polyclonal Antibody to PSD-93
    • Product Code: APZ-002
    • Short Description: A Rabbit Polyclonal Antibody to PSD-93
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Discs large homolog 2, Dlg2, Chapsyn-110
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