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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • BCTC
    A Potent Antagonist of TRPV1 and TRPM8 Channels
    • Product Code: B-110
    • Short Description: A Potent Antagonist of TRPV1 and TRPM8 Channels
    • CAS No.: 393514-24-4
    • MW: 372.9
  • BDNF Blocking Peptide
    A Blocking Peptide for Anti-BDNF Antibody
    • Product Code: BLP-NT010
    • Short Description: A Blocking Peptide for Anti-BDNF Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: BDNF
  • BDS-I
    A blocker of KV potassium channels and a modulator that slows the inactivation of NaV sodium channels (particularly NaV1.7)
    • Product Code: STB-400
    • Short Description: A blocker of KV potassium channels and a modulator that slows the inactivation of NaV sodium channels (particularly NaV1.7)
    • Applications: Electrophysiology
    • CAS No.: 207621-38-3
    • MW: 4708 Da
  • BDS-II
    A potent gating modifier/blocker of KV3 potassium channels and a modulator of NaV1.7 sodium channels
    • Product Code: B-450
    • Short Description: A potent gating modifier/blocker of KV3 potassium channels and a modulator of NaV1.7 sodium channels
    • Applications: Electrophysiology
    • MW: 4776.4 Da
  • BeKm-1
    A potent and selective blocker of hERG (KV11.1) potassium channels
    • Product Code: STB-470
    • Short Description: A potent and selective blocker of hERG (KV11.1) potassium channels
    • Applications: Electrophysiology
    • CAS No.: 524962-01-4
    • MW: 4092 Da
  • Bestrophin-1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Bestrophin-1 (extracellular) Antibody
    • Product Code: BLP-BC001
    • Short Description: A Blocking Peptide for Anti-Bestrophin-1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: BEST1
  • Bestrophin-2 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Bestrophin-2 (extracellular) Antibody
    • Product Code: BLP-BC002
    • Short Description: A Blocking Peptide for Anti-Bestrophin-2 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: BEST2
  • Bilobalide
    An Antagonist of 5-HT3A and GABA(A) Receptors
    • Product Code: B-145
    • Short Description: An Antagonist of 5-HT3A and GABA(A) Receptors
    • CAS No.:  33570-04-6
    • MW: 326.3
  • BmKI Toxin
    A potent activator/modulator that delays the fast inactivation of NaV1.5, NaV1.6, and NaV1.3 voltage-gated sodium channels
    • Product Code: STB-100
    • Short Description: A potent activator/modulator that delays the fast inactivation of NaV1.5, NaV1.6, and NaV1.3 voltage-gated sodium channels
    • Applications: Electrophysiology
    • MW: 7217 Da
  • Bombesin
    A potent agonist for the Bombesin (BB1, BB2, and BB3) G-protein coupled receptors
    • Product Code: GPB-100
    • Short Description: A potent agonist for the Bombesin (BB1, BB2, and BB3) G-protein coupled receptors
    • Applications: Calcium imaging assay
    • CAS No.: 31362-50-2
    • MW: 1620.8 Da
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