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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

974 Results
  • human GALP (3-32)
    Bioassay Tested
    human GALP (3-32)
    A non-selective and potent agonist of Galanin receptors
    • Product Code: GPG-250
    • Short Description: A non-selective and potent agonist of Galanin receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • MW: 3102 Da
  • Human GPR55 (extracellular) Blocking Peptide
    Human GPR55 (extracellular) Blocking Peptide
    A Blocking peptide for Anti-Human GPR55 (extracellular) Antibody
    • Product Code: BLP-CR061
    • Short Description: A Blocking peptide for Anti-Human GPR55 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR55
  • human proBDNF-Biotin
    Bioassay Tested
    human proBDNF-Biotin
    A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Product Code: B-257-B
    • Short Description: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Label: Biotin
    • Applications: Cell Culture, In vitro binding assay, Western blot, Live imaging
    • MW: ~52.5 kDa (dimer)
  • human proBDNF-Biotin
    Bioassay Tested
    human proBDNF-Biotin
    A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Product Code: B-256-B
    • Short Description: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Label: Biotin
    • Applications: Cell Culture, Western blot, Live imaging
    • MW: ~52 kDa (dimer)
  • Human TrkB-Fc Chimera
    Bioassay Tested
    Human TrkB-Fc Chimera
    A recombinant human TrkB-Fc fusion protein that acts as an inhibitor of BDNF and NT-4 activity, preventing their binding to native TrkB receptors
    • Product Code: RPC-001
    • Short Description: A recombinant human TrkB-Fc fusion protein that acts as an inhibitor of BDNF and NT-4 activity, preventing their binding to native TrkB receptors
    • Applications: Cell Culture, Western blot
    • MW: ~120 kDa
  • Human TRPV1 (extracellular) Blocking Peptide
    Human TRPV1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Human TRPV1 (extracellular) Antibody
    • Product Code: BLP-CC334
    • Short Description: A Blocking Peptide for Anti-Human TRPV1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: TRPV1
  • Human TRPV2/VRL1 (extracellular) Blocking Peptide
    Human TRPV2/VRL1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Human TRPV2/VRL1 (extracellular) Antibody
    • Product Code: BLP-CC139
    • Short Description: A Blocking Peptide for Anti-Human TRPV2/VRL1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: TRPV2
  • human, rat Spexin-1
    Bioassay Tested
    human, rat Spexin-1
    A potent agonist of Galanin 2 and Galanin 3 receptors
    • Product Code: SPS-100
    • Short Description: A potent agonist of Galanin 2 and Galanin 3 receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 1370290-58-6
    • MW: 1620 Da
  • Huwentoxin-I
    Bioassay Tested
    Huwentoxin-I
    A presynaptic blocker of N-type (CaV2.2) calcium channels, TTX-sensitive (NaV) sodium, and muscle nAChR channels
    • Product Code: STH-050
    • Short Description: A presynaptic blocker of N-type (CaV2.2) calcium channels, TTX-sensitive (NaV) sodium, and muscle nAChR channels
    • Applications: Electrophysiology
    • MW: 3750 Da
  • Huwentoxin-XVI
    Bioassay Tested
    Huwentoxin-XVI
    A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Product Code: STH-105
    • Short Description: A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Applications: Electrophysiology
    • CAS No.: 1600543-88-1
    • MW: 4437 Da
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