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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-ADAM22 (extracellular) Antibody
    Anti-ADAM22 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ADAM22
    • Product Code: ANR-120
    • Short Description: A Rabbit Polyclonal Antibody to ADAM22
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular) Antibody
    Anti-Adenosine A2A Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-008
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-PE Antibody
    Anti-Adenosine A2A Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: AAR-008-PE
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2A</sub> Receptor Antibody
    KO Validate'
    Anti-Adenosine A2A Receptor Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-002
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Alpha 2 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 2 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase
    • Product Code: ANP-002
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2
  • Anti-Alpha 3 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 3 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase
    • Product Code: ANP-003
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3
  • Anti-ASIC1 Antibody
    KO Validate'
    Anti-ASIC1 Antibody
    A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Product Code: ASC-014
    • Short Description: A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • Anti-ASIC2a Antibody
    KO Validate'
    Anti-ASIC2a Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Product Code: ASC-012
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC2a-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-ASIC2a-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
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