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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-ASIC3 Antibody
    KO Validate'
    Anti-ASIC3 Antibody
    A Rabbit Polyclonal Antibody to ASIC3 Channel
    • Product Code: ASC-018
    • Short Description: A Rabbit Polyclonal Antibody to ASIC3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
  • Anti-ASIC5 (extracellular) Antibody
    Anti-ASIC5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Product Code: ASC-032
    • Short Description: A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
  • Anti-BDNF Antibody
    KO Validate'
    Anti-BDNF Antibody
    A Rabbit Polyclonal Antibody to BDNF
    • Product Code: ANT-010
    • Short Description: A Rabbit Polyclonal Antibody to BDNF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Anti-Bestrophin-1 (extracellular) Antibody
    Anti-Bestrophin-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Product Code: ABC-001
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1
  • Anti-Bestrophin-2 (extracellular) Antibody
    Anti-Bestrophin-2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Product Code: ABC-002
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1
  • Anti-Bombesin Receptor 1 (NMBR) Antibody
    Anti-Bombesin Receptor 1 (NMBR) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor
    • Product Code: ABR-004
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor
  • Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody
    Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor
    • Product Code: ABR-002
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor
  • Anti-CACNA1A (Ca<sub>V</sub>2.1) Antibody
    KO Validate'
    Anti-CACNA1A (CaV2.1) Antibody
    A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Product Code: ACC-001
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI
  • Anti-CACNA1B (Ca<sub>V</sub>2.2) Antibody
    KO Validate'
    Anti-CACNA1B (CaV2.2) Antibody
    A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Product Code: ACC-002
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII
  • Anti-CACNB1 Antibody
    Anti-CACNB1 Antibody
    A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit
    • Product Code: ACC-106
    • Short Description: A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1
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