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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • µ-Conotoxin SxIIIA
    Bioassay Tested
    µ-Conotoxin SxIIIA
    A potent blocker of the skeletal muscle NaV1.4 channel, with moderate activity on neuronal NaV1.1, NaV1.2, and NaV1.6 channels
    • Product Code: STC-645
    • Short Description: A potent blocker of the skeletal muscle NaV1.4 channel, with moderate activity on neuronal NaV1.1, NaV1.2, and NaV1.6 channels
    • Applications: Electrophysiology
    • MW: 2271.7 Da
  • µ-Opioid Receptor/OPRM1 (extracellular) Blocking Peptide
    µ-Opioid Receptor/OPRM1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-µ-Opioid Receptor/OPRM1 (extracellular) Antibody
    • Product Code: BLP-OR011
    • Short Description: A Blocking Peptide for Anti-µ-Opioid Receptor/OPRM1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: OPRM1
  • µ-TRTX-Tsp1a
    Bioassay Tested
    µ-TRTX-Tsp1a
    A potent blocker of NaV1.7 channels
    • Product Code: STT-500
    • Short Description: A potent blocker of NaV1.7 channels
    • Applications: Electrophysiology
    • MW: 3391 Da
  • µ/ω-TRTX-Tap1a
    Bioassay Tested
    µ/ω-TRTX-Tap1a
    A potent blocker/gating-modifier of multiple NaV and T-type (CaV3.1, CaV3.2) channels
    • Product Code: STT-600
    • Short Description: A potent blocker/gating-modifier of multiple NaV and T-type (CaV3.1, CaV3.2) channels
    • Applications: Electrophysiology
    • MW: 4182.7 Da
  • ɣ-Dendrotoxin
    Bioassay Tested
    ɣ-Dendrotoxin
    A potent blocker of KCa1.1 (BK) K+ channels and KV1.1, KV1.2 potassium channels
    • Product Code: D-370
    • Short Description: A potent blocker of KCa1.1 (BK) K+ channels and KV1.1, KV1.2 potassium channels
    • Applications: Electrophysiology
    • MW: ~7000 Da
  • ɩ-Conotoxin RXIA
    Bioassay Tested
    ɩ-Conotoxin RXIA
    An activator of NaV1.6, NaV1.2, and NaV1.7 channels
    • Product Code: STI-300
    • Short Description: An activator of NaV1.6, NaV1.2, and NaV1.7 channels
    • Applications: Electrophysiology
    • MW: 4975.6 Da
  • α-Asarone
    Bioassay Tested
    α-Asarone
    An Antagonist of NaV Channels, GABA(A) Receptors, NMDA Receptors and an Inhibitor of TrkB Receptor
    • Product Code: A-260
    • Short Description: An Antagonist of NaV Channels, GABA(A) Receptors, NMDA Receptors and an Inhibitor of TrkB Receptor
    • CAS No.: 2883-98-9
    • MW: 208.3
  • α-Bungarotoxin
    Bioassay Tested
    α-Bungarotoxin
    A potent antagonist of muscle nAChRs, α7 nAChRs, and GABA(A) receptor subtypes
    • Product Code: B-100
    • Short Description: A potent antagonist of muscle nAChRs, α7 nAChRs, and GABA(A) receptor subtypes
    • Applications: Electrophysiology
    • CAS No.: 11032-79-4
    • MW: 7984 Da
  • α-Bungarotoxin-ATTO Fluor-488
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: ATTO 488
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining
    • MW: ~9140 Da
  • α-Bungarotoxin-ATTO Fluor-633
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-633
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-FR
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: ATTO 633
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~9140 Da
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