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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-LGI1 Antibody
    Anti-LGI1 Antibody
    A Rabbit Polyclonal Antibody to LGI1
    • Product Code: ALR-041
    • Short Description: A Rabbit Polyclonal Antibody to LGI1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT
  • Anti-MC3 Receptor (extracellular) Antibody
    Anti-MC3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Product Code: AMR-023
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 3, MC3R, MC3-R
  • Anti-MC4 Receptor (extracellular) Antibody
    KO Validate'
    Anti-MC4 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Product Code: AMR-024
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 4, MC4R, MC4-R
  • Anti-MCHR1 (extracellular) Antibody
    Anti-MCHR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1
    • Product Code: AMR-041
    • Short Description: A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1
  • Anti-mGluR1 (extracellular) Antibody
    Anti-mGluR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1
    • Product Code: AGC-006
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Target: Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13
  • Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGC-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13
  • Anti-mGluR2 (extracellular) Antibody
    Anti-mGluR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2
    • Product Code: AGC-011
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B
  • Anti-mGluR3 (extracellular) Antibody
    KO Validate'
    Anti-mGluR3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3
    • Product Code: AGC-012
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C
  • Anti-mGluR4 (extracellular) Antibody
    Anti-mGluR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4
    • Product Code: AGC-014
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D
  • Anti-mGluR5 (extracellular) Antibody
    Anti-mGluR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 5
    • Product Code: AGC-007
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
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