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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

970 Results
  • Anti-PSD-95 Antibody
    Anti-PSD-95 Antibody
    A Rabbit Polyclonal Antibody to PSD-95
    • Product Code: APZ-009
    • Short Description: A Rabbit Polyclonal Antibody to PSD-95
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15
  • Anti-RAMP1 (extracellular) Antibody
    KO Validate'
    Anti-RAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP1
    • Product Code: ARR-021
    • Short Description: A Rabbit Polyclonal Antibody to RAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1
  • Anti-RIM1 Antibody
    Anti-RIM1 Antibody
    A Rabbit Polyclonal Antibody to RIM1
    • Product Code: AIP-014
    • Short Description: A Rabbit Polyclonal Antibody to RIM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1
  • Anti-SAP-97/DLG1 Antibody
    Anti-SAP-97/DLG1 Antibody
    A Rabbit Polyclonal Antibody to SAP-97
    • Product Code: APZ-010
    • Short Description: A Rabbit Polyclonal Antibody to SAP-97
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Synapse-associated protein 97, Discs large homolog 1
  • Anti-SAP102 Antibody
    Anti-SAP102 Antibody
    A Rabbit Polyclonal Antibody to SAP-102
    • Product Code: APZ-003
    • Short Description: A Rabbit Polyclonal Antibody to SAP-102
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Synapse-associated protein 102, SAP-102, Discs large homolog 3, DLG3, NE-DLG
  • Anti-SCN1A (Na<sub>V</sub>1.1) (extracellular) Antibody
    Anti-SCN1A (NaV1.1) (extracellular) Antibody
    A Rabbit polyclonal antibody to Nav1.1 (extracellular)
    • Product Code: ASC-051
    • Short Description: A Rabbit polyclonal antibody to Nav1.1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium channel protein type I subunit alpha, NAC1
  • Anti-SCN1A (Na<sub>V</sub>1.1) Antibody
    KO Validate'
    Anti-SCN1A (NaV1.1) Antibody
    A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Product Code: ASC-001
    • Short Description: A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
  • Anti-SCN1A (Na<sub>V</sub>1.1)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-001-AR
    • Short Description: A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
  • Anti-SCN2A (Na<sub>V</sub>1.2) Antibody
    KO Validate'
    Anti-SCN2A (NaV1.2) Antibody
    A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel
    • Product Code: ASC-002
    • Short Description: A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
  • Anti-SCN2A (Na<sub>V</sub>1.2)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-SCN2A (NaV1.2)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-002-AR
    • Short Description: A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
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