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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

401 Results
  • Anti-K<sub>V</sub>6.4 (KCNG4) Antibody
    Anti-KV6.4 (KCNG4) Antibody
    A Rabbit Polyclonal antibody to Kv6.4 (KCNG4)
    • Product Code: APC-138
    • Short Description: A Rabbit Polyclonal antibody to Kv6.4 (KCNG4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Potassium Voltage-Gated Channel Subfamily G Member 4, Voltage-gated potassium channel subunit Kv6.4
  • Anti-K<sub>V</sub>8.1 (KCNV1) Antibody
    Anti-KV8.1 (KCNV1) Antibody
    A Rabbit Polyclonal antibody to KV8.1 (KCNV1)
    • Product Code: APC-139
    • Short Description: A Rabbit Polyclonal antibody to KV8.1 (KCNV1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Potassium Voltage-Gated Channel Subfamily V Member 1
  • Anti-K<sub>V</sub>β1 Antibody
    Anti-KVβ1 Antibody
    A Rabbit Polyclonal Antibody to KVβ1 Subunit
    • Product Code: APC-127
    • Short Description: A Rabbit Polyclonal Antibody to KVβ1 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1
  • Anti-K<sub>V</sub>β2 Antibody
    Anti-KVβ2 Antibody
    A Rabbit Polyclonal Antibody to KV?2 Subunit
    • Product Code: APC-117
    • Short Description: A Rabbit Polyclonal Antibody to KV?2 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated potassium channel subunit beta-2, KV beta 2, KCNAB2, Ckbeta2
  • Anti-LGI1 Antibody
    Anti-LGI1 Antibody
    A Rabbit Polyclonal Antibody to LGI1
    • Product Code: ALR-041
    • Short Description: A Rabbit Polyclonal Antibody to LGI1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT
  • Anti-LRRC8A (extracellular) Antibody
    Anti-LRRC8A (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LRRC8A
    • Product Code: AAC-001
    • Short Description: A Rabbit Polyclonal Antibody to LRRC8A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence
    • Target: Leucine-rich repeat-containing protein 8A, Volume-regulated anion channel subunit LRRC8A, LRRC8, Swelling protein 1, SWELL1
  • Anti-Mouse K<sub>V</sub>1.7 (KCNA7) Antibody
    Anti-Mouse KV1.7 (KCNA7) Antibody
    A Rabbit Polyclonal Antibody to KV1.7 (KCNA7) Channel
    • Product Code: APC-063
    • Short Description: A Rabbit Polyclonal Antibody to KV1.7 (KCNA7) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Western blot
    • Target: Potassium voltage-gated channel subfamily A member 7
  • Anti-MRS2 Antibody
    Anti-MRS2 Antibody
    A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter
    • Product Code: ANT-148
    • Short Description: A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L
  • Anti-Na<sub>V</sub>1.5 (SCN5A) (1978-2016) Antibody
    Anti-NaV1.5 (SCN5A) (1978-2016) Antibody
    A Rabbit Polyclonal Antibody to NaV1.5 (SCN5A) (1978-2016) Channel
    • Product Code: ASC-013
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.5 (SCN5A) (1978-2016) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunohistochemistry
    • Target: Sodium channel protein type 5 subunit alpha, SKM2
  • Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Product Code: AGC-002
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
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