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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

401 Results
  • Anti-GFAP-ATTO Fluor-647N Antibody
    Anti-GFAP-ATTO Fluor-647N Antibody
    A Rabbit Polyclonal Antibody to GFAP conjugated to the fluorescent dye ATTO Fluor-647N
    • Product Code: AFP-001-FRN
    • Short Description: A Rabbit Polyclonal Antibody to GFAP conjugated to the fluorescent dye ATTO Fluor-647N
    • Host: Rabbit
    • Label: ATTO 647N
    • Reactivity: Rat, Mouse
    • Applications: Immunocytochemistry, Immunohistochemistry
    • Target: Glial fibrillary acidic protein
  • Anti-GIPR (extracellular) Antibody
    Anti-GIPR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Product Code: AGR-025
    • Short Description: A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor
  • Anti-GLP1R (extracellular) Antibody
    KO Validate'
    Anti-GLP1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Product Code: AGR-021
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular)-FITC Antibody
    Anti-GLP1R (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular)-PE Antibody
    Anti-GLP1R (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Product Code: AGR-021-PE
    • Short Description: A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-HCN3 (extracellular) Antibody
    Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN3 Antibody
    Anti-HCN3 Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-057
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN4 Antibody
    KO Validate'
    Anti-HCN4 Antibody
    A Rabbit Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052
    • Short Description: A Rabbit Polyclonal Antibody to HCN4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Anti-Human P2Y11 Receptor Antibody
    Anti-Human P2Y11 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Product Code: APR-015
    • Short Description: A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Target: P2Y purinoceptor 11, P2RY11
  • Anti-KChIP1 (KCNIP1) Antibody
    Anti-KChIP1 (KCNIP1) Antibody
    A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Product Code: APC-141
    • Short Description: A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1
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