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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

401 Results
  • Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGC-002-AG
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Anti-NMDAR2B (GluN2B) (extracellular) Antibody
    KO Validate'
    Anti-NMDAR2B (GluN2B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)
    • Product Code: AGC-003
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
  • Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGC-003-AR
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
  • Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Product Code: AGC-018
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2C, NR2C, GluN2C, Ionotropic glutamate receptor subunit ε3, N-methyl-D-aspartate receptor subunit 2C
  • Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
    Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2D (GRIN2D)
    • Product Code: AGC-020
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2D (GRIN2D)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D
  • Anti-NMDAR3A (GRIN3A) (extracellular) Antibody
    Anti-NMDAR3A (GRIN3A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 3A (GRIN3A)
    • Product Code: AGC-030
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 3A (GRIN3A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: NR3A, NMDAR-L, GluN3A, Ionotropic glutamate receptor NMDA 3A, N-methyl-D-aspartate subunit 3A
  • Anti-NMDAR3B (GRIN3B) (extracellular) Antibody
    Anti-NMDAR3B (GRIN3B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 3B (GRIN3B)
    • Product Code: AGC-031
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 3B (GRIN3B)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: NR3B, GluN3B, Ionotropic glutamate receptor NMDA 3B, N-methyl-D-aspartate subunit 3B
  • Anti-P2X1 Receptor (extracellular) Antibody
    Anti-P2X1 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Product Code: APR-022
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
  • Anti-P2X1 Receptor (extracellular)-APC Antibody
    Anti-P2X1 Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APR-022-APC
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2RX1, P2X purinoceptor 1, ATP Receptor
  • Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-022-AG
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
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