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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

401 Results
  • Anti-<em>slo</em>β4 (KCNMB4) Antibody
    Anti-sloβ4 (KCNMB4) Antibody
    A Rabbit Polyclonal Antibody to slo?4 (KCNMB4) Subunit
    • Product Code: APC-061
    • Short Description: A Rabbit Polyclonal Antibody to slo?4 (KCNMB4) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4
  • Anti-TMEM175 Antibody
    Anti-TMEM175 Antibody
    A Rabbit Polyclonal antibody to TMEM175
    • Product Code: APC-175
    • Short Description: A Rabbit Polyclonal antibody to TMEM175
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175
  • Anti-TRPC7 (extracellular) Antibody
    Anti-TRPC7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPC7 Channel
    • Product Code: ACC-066
    • Short Description: A Rabbit Polyclonal Antibody to TRPC7 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Transient receptor protein 7, TRP7
  • Anti-TRPM4 Antibody
    KO Validate'
    Anti-TRPM4 Antibody
    A Rabbit Polyclonal Antibody to TRPM4 Channel
    • Product Code: ACC-044
    • Short Description: A Rabbit Polyclonal Antibody to TRPM4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1
  • Anti-TRPM6 (extracellular) Antibody
    Anti-TRPM6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM6 Channel
    • Product Code: ACC-046
    • Short Description: A Rabbit Polyclonal Antibody to TRPM6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6
  • Anti-TRPM6 (extracellular)-FITC Antibody
    Anti-TRPM6 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPM6 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-046-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPM6 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6
  • Anti-TRPM7 (extracellular) Antibody
    Anti-TRPM7 (extracellular) Antibody
    A Rabbit Polyclonal antibody to TRPM7 (extracellular)
    • Product Code: ACC-129
    • Short Description: A Rabbit Polyclonal antibody to TRPM7 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 7, Long transient receptor potential channel 7, LTRPC7, Channel-kinase 1, CHAK1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK
  • Anti-TRPM7 (extracellular)-FITC Antibody
    Anti-TRPM7 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPM7 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-129-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPM7 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily M member 7, Long transient receptor potential channel 7, LTRPC7, Channel-kinase 1, CHAK1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK
  • Anti-TRPM7 Antibody
    KO Validate'
    Anti-TRPM7 Antibody
    A Rabbit Polyclonal Antibody to TRPM7 Channel
    • Product Code: ACC-047
    • Short Description: A Rabbit Polyclonal Antibody to TRPM7 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: LTrpC7, TRP-PLIK, Channel-kinase 1, CHAK1, ALSPDC
  • Apamin
    Bioassay Tested
    Apamin
    A blocker of small conductance Ca2+-Activated K+ Channels (SK Type, KCa2)
    • Product Code: STA-200
    • Short Description: A blocker of small conductance Ca2+-Activated K+ Channels (SK Type, KCa2)
    • Applications: Electrophysiology
    • CAS No.: 24345-16-2
    • MW: 2027.3 Da
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