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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

401 Results
  • Anti-Angiotensin-(1-7) Mas Receptor Antibody
    KO Validate'
    Anti-Angiotensin-(1-7) Mas Receptor Antibody
    A Rabbit Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor
    • Product Code: AAR-013
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Proto-oncogene Mas, MAS1
  • Anti-Aquaporin 4 (AQP4) (249-323) Antibody
    KO Validate'
    Anti-Aquaporin 4 (AQP4) (249-323) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 (249-323) Channel
    • Product Code: AQP-004
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 (249-323) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Product Code: AQP-014
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AQP-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-ASCT1 (SLC1A4) Antibody
    Anti-ASCT1 (SLC1A4) Antibody
    A Rabbit Polyclonal Antibody to ASCT1 Transporter
    • Product Code: ANT-081
    • Short Description: A Rabbit Polyclonal Antibody to ASCT1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Alanine/serine/cysteine/threonine transporter 1, Neutral amino acid transporter A, SATT
  • Anti-ASIC2a Antibody
    KO Validate'
    Anti-ASIC2a Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Product Code: ASC-012
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC2a-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-ASIC2a-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC4 Antibody
    Anti-ASIC4 Antibody
    A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Product Code: ASC-015
    • Short Description: A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4
  • Anti-ASIC5 (extracellular) Antibody
    Anti-ASIC5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Product Code: ASC-032
    • Short Description: A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
  • Anti-CACNA1A (Ca<sub>V</sub>2.1) Antibody
    KO Validate'
    Anti-CACNA1A (CaV2.1) Antibody
    A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Product Code: ACC-001
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI
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