Synaptic Function

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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226 Results
  • Anti-Connexin-29 (extracellular) Antibody
    Anti-Connexin-29 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Connexin-29
    • Product Code: ACC-223
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-29
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX29, Gap junction γ3 protein, Gap junction ε1 protein, GJC3, GJE1
  • Anti-Connexin-31 (GJB3) Antibody
    Anti-Connexin-31 (GJB3) Antibody
    A Rabbit Polyclonal Antibody to Connexin-31
    • Product Code: ACC-213
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-31
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX31, Gap junction β3 protein
  • Anti-Connexin-36 Antibody
    Anti-Connexin-36 Antibody
    A Rabbit Polyclonal Antibody to Connexin-36
    • Product Code: ACC-209
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-36
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
  • Anti-Connexin-36-ATTO Fluor-488 Antibody
    Anti-Connexin-36-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Connexin-36 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-209-AG
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-36 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
  • Anti-Connexin-45 (GJC1) Antibody
    Anti-Connexin-45 (GJC1) Antibody
    A Rabbit Polyclonal Antibody to Connexin-45
    • Product Code: ACC-207
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-45
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7
  • Anti-Connexin-47 (GJC2) Antibody
    Anti-Connexin-47 (GJC2) Antibody
    A Rabbit Polyclonal antibody to Connexin-47 (GJC2)
    • Product Code: ACC-222
    • Short Description: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6
  • Anti-GPR52 Antibody
    Anti-GPR52 Antibody
    A Rabbit Polyclonal Antibody to GPR52
    • Product Code: AGR-058
    • Short Description: A Rabbit Polyclonal Antibody to GPR52
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 52
  • Anti-Homer1 Antibody
    Anti-Homer1 Antibody
    A Rabbit Polyclonal Antibody to Homer1
    • Product Code: APZ-026
    • Short Description: A Rabbit Polyclonal Antibody to Homer1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47
  • Anti-Human ZACN (extracellular) Antibody
    Anti-Human ZACN (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Product Code: AZC-001
    • Short Description: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
  • Anti-NALCN/VGCNL1 (extracellular) Antibody
    Anti-NALCN/VGCNL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Product Code: ASC-022
    • Short Description: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
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