Synaptic Function

Synaptic Function

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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226 Results
  • Anti-PICK1 Antibody
    Anti-PICK1 Antibody
    A Rabbit Polyclonal Antibody to PICK1
    • Product Code: APZ-014
    • Short Description: A Rabbit Polyclonal Antibody to PICK1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein
  • Anti-PSD-95 Antibody
    Anti-PSD-95 Antibody
    A Rabbit Polyclonal Antibody to PSD-95
    • Product Code: APZ-009
    • Short Description: A Rabbit Polyclonal Antibody to PSD-95
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15
  • Anti-RIM1 Antibody
    Anti-RIM1 Antibody
    A Rabbit Polyclonal Antibody to RIM1
    • Product Code: AIP-014
    • Short Description: A Rabbit Polyclonal Antibody to RIM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1
  • Anti-SCN2B (Na<sub>V</sub>β<sub>2</sub>) Antibody
    KO Validate'
    Anti-SCN2B (NaVβ2) Antibody
    A Rabbit Polyclonal Antibody to NaVβ2 Subunit
    • Product Code: ASC-007
    • Short Description: A Rabbit Polyclonal Antibody to NaVβ2 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel subunit beta-2
  • Anti-SCN7A (Na<sub>X</sub>) Antibody
    Anti-SCN7A (NaX) Antibody
    A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Product Code: ASC-050
    • Short Description: A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium Channel Protein Type 7 Subunit Alpha, Putative voltage-gated sodium channel subunit alpha Nax, SCN6A, Nav2.1, Nav2.2
  • Anti-Semaphorin 6A (extracellular) Antibody
    Anti-Semaphorin 6A (extracellular) Antibody
    A Rabbit Polyclonal antibody to Semaphorin 6A
    • Product Code: ASR-071
    • Short Description: A Rabbit Polyclonal antibody to Semaphorin 6A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SEMAQ, SEMA6A-1, SEMA6A
  • Anti-Shank1 Antibody
    Anti-Shank1 Antibody
    A Rabbit Polyclonal Antibody to Shank1
    • Product Code: APZ-011
    • Short Description: A Rabbit Polyclonal Antibody to Shank1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon
  • Anti-Shank3 Antibody
    Anti-Shank3 Antibody
    A Rabbit Polyclonal Antibody to Shank3
    • Product Code: APZ-013
    • Short Description: A Rabbit Polyclonal Antibody to Shank3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2
  • Anti-SLC17A5 (Sialin) Antibody
    Anti-SLC17A5 (Sialin) Antibody
    A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter
    • Product Code: AST-015
    • Short Description: A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter
  • Anti-Slitrk1 (extracellular) Antibody
    KO Validate'
    Anti-Slitrk1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Slitrk1
    • Product Code: ASR-081
    • Short Description: A Rabbit Polyclonal Antibody to Slitrk1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12
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