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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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1413 Results
  • Tetrodotoxin citrate
    Bioassay Tested
    Tetrodotoxin citrate
    Tetrodotoxin citrate (#T-550) is a highly pure, natural, and biologically active compound.
    • Product Code: T-550
    • Applications: Electrophysiology
    • CAS No.: 18660-81-6
    • MW: 319.27
  • Anti-SCN1A (Na<sub>V</sub>1.1) Antibody
    KO Validate'
    Anti-SCN1A (NaV1.1) Antibody
    A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Product Code: ASC-001
    • Short Description: A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular)-PE Antibody
    Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye Phycoerythrin
    • Product Code: APC-101-PE
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye Phycoerythrin
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular) Antibody
    KO Validate'
    Anti-KV1.3 (KCNA3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Product Code: APC-101
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-TrkA (extracellular) Antibody
    KO Validate'
    Anti-TrkA (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TrkA
    • Product Code: ANT-018
    • Short Description: A Rabbit Polyclonal Antibody to TrkA
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
  • Iberiotoxin-ATTO Fluor-647N
    Bioassay Tested
    Iberiotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Product Code: STI-400-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4858 Da
  • Tertiapin-Q-ATTO Fluor-633
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-633
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-FR
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Applications: Electrophysiology, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 3159.6 Da
  • Tertiapin-Q-ATTO Fluor-488
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: 3195.5 Da
  • Stichodactyla Toxin-Biotin
    Bioassay Tested
    Stichodactyla Toxin-Biotin
    A biotinylated probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Product Code: STS-400-B
    • Applications: Electrophysiology, Immunofluorescence, Indirect flow cytometry
    • MW: 4563 Da
  • Stichodactyla Toxin-ATTO Fluor-590
    Bioassay Tested
    Stichodactyla Toxin-ATTO Fluor-590
    A fluorescent probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Product Code: STS-400-AR
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: ~4627 Da
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