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The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.
- If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
  • Heat the samples at 70°C for 10 min.
  • Increase the transfer time.
- If the band’s MW is above the expected MW, it could be due to post-translational modifications.
- In either event, you should use a blocking peptide as a negative control.
Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.
1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.
Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.
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Phrixotoxin-2

A potent blocker/gating modifier of KV4.2 and KV4.3 channels
- Product Code: STP-710
- Short Description: A potent blocker/gating modifier of K_V4.2 and K_V4.3 channels
- Applications: Electrophysiology
- CAS No.: 221889-63-0
- MW: 3921.7 Da
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Phrixotoxin-1

A potent blocker/gating modifier of KV4.2 and KV4.3 channels
- Product Code: STP-700
- Short Description: A potent blocker/gating modifier of K_V4.2 and K_V4.3 channels
- Applications: Electrophysiology
- CAS No.: 221872-97-5
- MW: 3548.4 Da
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Pandinotoxin Kα

A blocker of KV1.2, KV3, KV1.4 and KV2 K+ channels
- Product Code: STP-500
- Applications: Electrophysiology
- CAS No.: 185529-64-0
- MW: 4033 Da
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ProTx-I

A blocker/gating modifier of NaV, CaV3.1, KV2.1 and TRPA1 channels
- Product Code: STP-400
- Applications: Electrophysiology
- CAS No.: 484598-35-8
- MW: 3987.6 Da
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Phlo1b

A blocker/gating modifier of NaV1.7 channels
- Product Code: STP-370
- Short Description: A blocker/gating modifier of Na_V1.7 channels
- Applications: Electrophysiology
- MW: 4140.8 Da
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Phlo1a

A blocker/gating modifier of NaV1.7 channels
- Product Code: STP-350
- Short Description: A blocker/gating modifier of Na_V1.7 channels
- Applications: Electrophysiology
- MW: 4106.8 Da
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Psalmotoxin-1

A potent blocker of ASIC1a channels
- Product Code: STP-200
- Short Description: A potent blocker of ASIC1a channels
- Applications: Electrophysiology
- CAS No.: 316808-68-1
- MW: 4689.5 Da
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ProTx-III

A potent blocker/gating modifier of NaV1.7 channels
- Product Code: STP-150
- Short Description: A potent blocker/gating modifier of Na_V1.7 channels
- Applications: Electrophysiology
- MW: 3802.5 Da
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ProTx-II

A highly potent gating modifier of NaV1.7 channels with weaker activity on CaV3.2 T-type channels
- Product Code: STP-100
- Short Description: A highly potent gating modifier of Na_V1.7 channels with weaker activity on Ca_V3.2 T-type channels
- Applications: Electrophysiology
- CAS No.: 484598-36-9
- MW: 3826.6 Da
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Pe1b Toxin

A selective blocker/gating modifier of NaV1.7 channels
- Product Code: STP-050
- Short Description: A selective blocker/gating modifier of Na_V1.7 channels
- Applications: Electrophysiology
- MW: 3975.5 Da

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