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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

Category: Microglia Markers
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320 Results
  • Anti-CNTF Antibody
    Anti-CNTF Antibody
    A Rabbit Polyclonal Antibody to CNTF
    • Product Code: ANT-031
    • Short Description: A Rabbit Polyclonal Antibody to CNTF
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ciliary neurotrophic factor
  • Anti-CDNF Antibody
    Anti-CDNF Antibody
    A Rabbit Polyclonal Antibody to Cerebral Dopamine Neurotrophic Factor
    • Product Code: ANT-027
    • Short Description: A Rabbit Polyclonal Antibody to Cerebral Dopamine Neurotrophic Factor
    • Host: Rabbit
    • Reactivity: Human, Rat
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
  • Anti-proGDNF Antibody
    Anti-proGDNF Antibody
    A Rabbit Polyclonal Antibody to proGDNF
    • Product Code: ANT-026
    • Short Description: A Rabbit Polyclonal Antibody to proGDNF
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Glial cell line-derived neurotrophic factor precursor
  • Anti-GDNF Antibody
    Anti-GDNF Antibody
    A Rabbit Polyclonal Antibody to GDNF
    • Product Code: ANT-014
    • Short Description: A Rabbit Polyclonal Antibody to GDNF
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
  • Guinea pig Anti-BDNF Antibody
    Guinea pig Anti-BDNF Antibody
    A Guinea Pig Polyclonal Antibody to BDNF
    • Product Code: ANT-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to BDNF
    • Host: Guinea Pig
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Anti-BDNF Antibody
    KO Validate'
    Anti-BDNF Antibody
    A Rabbit Polyclonal Antibody to BDNF
    • Product Code: ANT-010
    • Short Description: A Rabbit Polyclonal Antibody to BDNF
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Guinea pig Anti-proBDNF Antibody
    Guinea pig Anti-proBDNF Antibody
    A Guinea Pig Polyclonal Antibody to proBDNF
    • Product Code: ANT-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proBDNF
    • Host: Guinea Pig
    • Reactivity: Human, Rat
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-proBDNF-ATTO Fluor-488 Antibody
    Anti-proBDNF-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-proBDNF Antibody
    Anti-proBDNF Antibody
    A Rabbit Polyclonal Antibody to proBDNF
    • Product Code: ANT-006
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Guinea pig Anti-proNGF Antibody
    Guinea pig Anti-proNGF Antibody
    A Guinea Pig Polyclonal Antibody to proNGF
    • Product Code: ANT-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proNGF
    • Host: Guinea Pig
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nerve growth factor precursor
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