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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

Category: Cardiovascular
954 Results
  • WRW4
    A Potent Antagonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Product Code: GPW-110
    • Short Description: A Potent Antagonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Applications: Calcium imaging assay
    • CAS No.: 878557-55-2
    • MW: 1104.3 Da
  • WKYMVm
    A Potent Agonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Product Code: GPW-105
    • Short Description: A Potent Agonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Applications: Calcium imaging assay
    • CAS No.: 187986-17-0
    • MW: 856.11 Da
  • WKYMVM
    A Potent Agonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Product Code: GPW-100
    • Short Description: A Potent Agonist of FPR2 and FPR3 G-Protein Coupled Receptors
    • Applications: Calcium imaging assay
    • CAS No.: 187986-11-4
    • MW: 856.11 Da
  • SLIGKV-NH2
    A Potent Agonist of PAR2, Derived from PAR2 Auto-Ligand Sequence
    • Product Code: GPS-100
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 190383-13-2
    • MW: 614.77 Da
  • MMK1
    A Potent and Selective Agonist of FPR2 G-Protein Coupled Receptors
    • Product Code: GPM-120
    • Short Description: A Potent and Selective Agonist of FPR2 G-Protein Coupled Receptors
    • Applications: Calcium imaging assay
    • CAS No.: 271246-66-3
    • MW: 1611 Da
  • α-MSH
    A Ligand of Melanocortin G-Protein Coupled Receptor
    • Product Code: GPM-100
    • Short Description: A Ligand of Melanocortin G-Protein Coupled Receptor
    • Applications: Melanin production assay
    • CAS No.: 581-05-5
    • MW: 1665 Da
  • N-Formyl-Met-Leu-Phe
    A Potent Agonist of FPR1 G-Protein Coupled Receptor
    • Product Code: GPF-100
    • Short Description: A Potent Agonist of FPR1 G-Protein Coupled Receptor
    • Applications: Calcium imaging assay
    • CAS No.: 59880-97-6
    • MW: 437.6 Da
  • Angiotensin III
    A Ligand of AT1 and AT2 G-Protein Coupled Receptors
    • Product Code: GPA-130
    • Short Description: A Ligand of AT1 and AT2 G-Protein Coupled Receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 13602-53-4
    • MW: 931.1 Da
  • Angiotensin II
    A Ligand of AT1 and AT2 G-Protein Coupled Receptors
    • Product Code: GPA-100
    • Short Description: A Ligand of AT1 and AT2 G-Protein Coupled Receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 4474-91-3
    • MW: 1046.2 Da
  • Geldanamycin
    A Powerful Anticancer Antibiotic
    • Product Code: G-500
    • Short Description: A Powerful Anticancer Antibiotic
    • CAS No.: 30562-34-6
    • MW: 560.6
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