Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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796 Results
  • Anti-CD56/NCAM1 (extracellular) Antibody
    KO Validate'
    Anti-CD56/NCAM1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 1 (CD56)
    • Product Code: ANR-041
    • Short Description: A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 1 (CD56)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Neural cell adhesion molecule 1, NCAM-1
  • Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Product Code: ANR-041-AG
    • Short Description: A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunohistochemistry
    • Target: NCAM-1, CD56
  • Anti-CD63 (extracellular) Antibody
    Anti-CD63 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular)
    • Product Code: ANR-210
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-APC Antibody
    Anti-CD63 (extracellular)-APC Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-210-APC
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-FITC Antibody
    Anti-CD63 (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-210-F
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-PE Antibody
    Anti-CD63 (extracellular)-PE Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Product Code: ANR-210-PE
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD73 (extracellular) Antibody
    Anti-CD73 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CD73
    • Product Code: ANT-066
    • Short Description: A Rabbit Polyclonal Antibody to CD73
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E
  • Anti-CD73 (extracellular)-FITC Antibody
    Anti-CD73 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-066-F
    • Short Description: A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E, ENT
  • Anti-CD81 (extracellular) Antibody
    Anti-CD81 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CD81 (extracellular)
    • Product Code: ANR-211
    • Short Description: A Rabbit Polyclonal antibody to CD81 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1
  • Anti-CD81 (extracellular)-APC Antibody
    Anti-CD81 (extracellular)-APC Antibody
    A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-211-APC
    • Short Description: A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1
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