Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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796 Results
  • Anti-ENaC α (SCNN1A) (extracellular) Antibody
    Anti-ENaC α (SCNN1A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaCα Channel
    • Product Code: ASC-030
    • Short Description: A Rabbit Polyclonal Antibody to ENaCα Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial sodium channel α subunit, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA
  • Anti-ENaC β (SCNN1B) (extracellular) Antibody
    Anti-ENaC β (SCNN1B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Product Code: ASC-019
    • Short Description: A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB
  • Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaC? Channel
    • Product Code: ASC-011
    • Short Description: A Rabbit Polyclonal Antibody to ENaC? Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG
  • Anti-Endothelin Receptor A (extracellular) Antibody
    Anti-Endothelin Receptor A (extracellular) Antibody
    A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Product Code: AER-004
    • Short Description: A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A
  • Anti-Endothelin Receptor B (extracellular) Antibody
    Anti-Endothelin Receptor B (extracellular) Antibody
    A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Product Code: AER-005
    • Short Description: A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, ETBR
  • Anti-ENPP1 (extracellular) Antibody
    Anti-ENPP1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to ENPP1 (extracellular)
    • Product Code: ANT-069
    • Short Description: A Rabbit Polyclonal antibody to ENPP1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Ectonucleotide Pyrophosphatase/ Phosphodiesterase Family Member 1, Plasma-Cell Membrane Glycoprotein PC-1, CD203a, Membrane Component Chromosome 6 Surface Marker 1, M6S1
  • Anti-EphA1 (extracellular) Antibody
    Anti-EphA1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA1 Receptor
    • Product Code: AER-011
    • Short Description: A Rabbit Polyclonal Antibody to EphA1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
  • Anti-EphA1 (extracellular)-FITC Antibody
    Anti-EphA1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AER-011-F
    • Short Description: A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
  • Anti-EphA2 (extracellular) Antibody
    Anti-EphA2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Product Code: AER-019
    • Short Description: A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2
  • Anti-EphA3 (extracellular) Antibody
    Anti-EphA3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA3 Receptor
    • Product Code: AER-013
    • Short Description: A Rabbit Polyclonal Antibody to EphA3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4
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