Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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796 Results
  • Anti-Adenosine A<sub>3</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-035-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA3, A3AR, Adenosine Receptor A3
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Product Code: AAR-043
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AAR-043-AG
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1
    • Product Code: AAR-011
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT1 Receptor, AT1R, AGTR1
  • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody
    Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: AAR-011-AO
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT1 Receptor, AT1R, AGTR1
  • Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
    Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: AT1 Receptor, AT1R, AGTR1
  • Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    KO Validate'
    Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2
    • Product Code: AAR-012
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT2 Receptor, AT2R, AGTR2, MRX88
  • Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AAR-012-AG
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT2 Receptor, AT2R, AGTR2, MRX88
  • Anti-Anoctamin-6 (extracellular) Antibody
    Anti-Anoctamin-6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Product Code: ACL-016
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
  • Anti-Anoctamin-6 (extracellular)-FITC Antibody
    Anti-Anoctamin-6 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACL-016-F
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
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