Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-PACC1/TMEM206 (extracellular) Antibody
    Anti-PACC1/TMEM206 (extracellular) Antibody
    A Rabbit Polyclonal antibody to PACC1/TMEM206 (extracellular)
    • Product Code: ACL-031
    • Short Description: A Rabbit Polyclonal antibody to PACC1/TMEM206 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Proton-Activated Chloride Channel, PAC, Transmembrane Protein 206, Proton Activated Chloride Channel 1, Acid-Sensitive Outwardly-Rectifying Anion Channel, ASOR
  • Anti-pan ASIC (extracellular) Antibody
    Anti-pan ASIC (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Product Code: ASC-031
    • Short Description: A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4
  • Anti-Pannexin 2 (extracellular) Antibody
    Anti-Pannexin 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Pannexin 2
    • Product Code: ACC-232
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: PANX2, PX2, hPANX2
  • Anti-PAR1 (F2R) (extracellular) Antibody
    Anti-PAR1 (F2R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Product Code: APR-036
    • Short Description: A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
  • Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Product Code: APR-036-F
    • Short Description: A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
  • Anti-PAR4 (F2RL3) (extracellular) Antibody
    KO Validate'
    Anti-PAR4 (F2RL3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4
    • Product Code: APR-034
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-PAR4 (F2RL3) (extracellular)-APC Antibody
    Anti-PAR4 (F2RL3) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to PAR4 (F2RL3) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APR-034-APC
    • Short Description: A Rabbit Polyclonal Antibody to PAR4 (F2RL3) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    KO Validate'
    Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-034-F
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-PepT2/SLC15A2 (extracellular) Antibody
    Anti-PepT2/SLC15A2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Product Code: APT-002
    • Short Description: A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter
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