Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody
    Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Renin Receptor
    • Product Code: AAR-014
    • Short Description: A Rabbit Polyclonal Antibody to Renin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2
  • Anti-Ret (extracellular) Antibody
    Anti-Ret (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ret Receptor
    • Product Code: ANT-025
    • Short Description: A Rabbit Polyclonal Antibody to Ret Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12
  • Anti-Ret Receptor (extracellular)-FITC Antibody
    Anti-Ret Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Ret receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-025-F
    • Short Description: A Rabbit Polyclonal Antibody to Ret receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51
  • Anti-RGMA (extracellular) Antibody
    Anti-RGMA (extracellular) Antibody
    A Rabbit polyclonal antibody to RGMA (extracellular).
    • Product Code: ANR-126
    • Short Description: A Rabbit polyclonal antibody to RGMA (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Repulsive Guidance Molecule A, RGM Domain Family Member A, Repulsive Guidance Molecule BMP Co-Receptor A, RGMa
  • Anti-Robo1 (extracellular) Antibody
    Anti-Robo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo1
    • Product Code: ANR-181
    • Short Description: A Rabbit Polyclonal Antibody to Robo1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-Robo1 (extracellular)-FITC Antibody
    Anti-Robo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-181-F
    • Short Description: A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-Robo2 (extracellular) Antibody
    Anti-Robo2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo2
    • Product Code: ANR-182
    • Short Description: A Rabbit Polyclonal Antibody to Robo2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Roundabout homolog 2, Roundabout Guidance Receptor 2
  • Anti-Robo3 (extracellular) Antibody
    Anti-Robo3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Robo3 (extracellular)
    • Product Code: ANR-183
    • Short Description: A Rabbit Polyclonal antibody to Robo3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Roundabout Homolog 3, Roundabout Guidance Receptor 3, Retinoblastoma-inhibiting gene 1 protein, RIG1
  • Anti-RXFP2 (extracellular) Antibody
    Anti-RXFP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Relaxin Receptor 2
    • Product Code: ARR-012
    • Short Description: A Rabbit Polyclonal Antibody to Relaxin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Relaxin receptor 2, G-protein coupled receptor affecting testicular descent, Leucine-rich repeat-containing G-protein coupled receptor 8, GPR106, GREAT, LGR8, INSL3R
  • Anti-S1PR1 (EDG1) (extracellular) Antibody
    Anti-S1PR1 (EDG1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Product Code: ASR-011
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
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