Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-SGLT2 (extracellular) Antibody
    Anti-SGLT2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Product Code: AGT-032
    • Short Description: A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2
  • Anti-SLC10A4 (extracellular) Antibody
    Anti-SLC10A4 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SLC10A4 (extracellular)
    • Product Code: ANT-174
    • Short Description: A Rabbit Polyclonal antibody to SLC10A4 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Sodium/Bile Acid Cotransporter 4, P4, Solute Carrier Family 10 Member 4
  • Anti-SLC11A1/NRAMP1 (extracellular) Antibody
    Anti-SLC11A1/NRAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1
    • Product Code: ANT-201
    • Short Description: A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
  • Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-201-F
    • Short Description: A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
  • Anti-SLC19A1/RFC1 (extracellular) Antibody
    Anti-SLC19A1/RFC1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC19A1 Transporter
    • Product Code: AFT-001
    • Short Description: A Rabbit Polyclonal Antibody to SLC19A1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1
  • Anti-SLC1A7 (extracellular) Antibody
    Anti-SLC1A7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)
    • Product Code: AGC-025
    • Short Description: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter
  • Anti-SLC22A3 (extracellular) Antibody
    Anti-SLC22A3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Organic Cation Transporter 3
    • Product Code: ACT-013
    • Short Description: A Rabbit Polyclonal Antibody to Organic Cation Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: OCT3, Organic cation transporter 3
  • Anti-SLC28A3/CNT3 (extracellular) Antibody
    Anti-SLC28A3/CNT3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC28A3/CNT3
    • Product Code: ANT-063
    • Short Description: A Rabbit Polyclonal Antibody to SLC28A3/CNT3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3
  • Anti-SLC35G1 (extracellular) Antibody
    Anti-SLC35G1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC35G1 Transporter
    • Product Code: ANT-196
    • Short Description: A Rabbit Polyclonal Antibody to SLC35G1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20
  • Anti-SLC3A1 (extracellular) Antibody
    Anti-SLC3A1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC3A1
    • Product Code: ANT-100
    • Short Description: A Rabbit Polyclonal Antibody to SLC3A1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1
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