Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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796 Results
  • Anti-C3aR1 (extracellular) Antibody
    Anti-C3aR1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Product Code: AAR-036
    • Short Description: A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C3aR1 (extracellular)-FITC Antibody
    Anti-C3aR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Product Code: AAR-036-V
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Host: Rabbit
    • Label: mFluor™ Violet 450
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C5aR2 (extracellular) Antibody
    Anti-C5aR2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to C5aR2 (extracellular).
    • Product Code: AAR-034
    • Short Description: A Rabbit Polyclonal antibody to C5aR2 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77
  • Anti-CACNA2D1 (Ca<sub>V</sub>α2δ1) (extracellular) Antibody
    KO Validate'
    Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Product Code: ACC-015
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
  • Anti-CACNA2D2 (Ca<sub>V</sub>α2δ2) (extracellular) Antibody
    Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Product Code: ACC-102
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-2
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular) Antibody
    Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit
    • Product Code: ACC-103
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular)-ATTO Fluor-594 Antibody
    Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-103-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D4 (Ca<sub>V</sub>α2δ4) (extracellular) Antibody
    Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit
    • Product Code: ACC-104
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
  • Anti-CACNA2D4 (Ca<sub>V</sub>α2δ4) (extracellular)-ATTO Fluor-594 Antibody
    Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-104-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
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