Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Mouse Anti-GIRK1 (K<sub>ir</sub>3.1) (extracellular) Antibody
    Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody
    A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel
    • Product Code: ALM-031
    • Short Description: A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: KCNJ3, G protein-activated inward rectifier potassium channel 1
  • Mouse Anti-Human Orai1 (extracellular) Antibody
    Mouse Anti-Human Orai1 (extracellular) Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Product Code: ALM-025
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody
    Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ALM-025-FR
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Host: Mouse
    • Label: ATTO 633
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Product Code: ALM-025-B
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Host: Mouse
    • Label: Biotin
    • Reactivity: Human
    • Applications: Western blot, Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) (extracellular) Antibody
    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody
    A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Product Code: ALM-051
    • Short Description: A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Mouse Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody
    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody
    A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ALM-051-AG
    • Short Description: A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Mouse
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Mouse Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) (extracellular)-Biotin Antibody
    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody
    A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to Biotin
    • Product Code: ALM-051-B
    • Short Description: A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to Biotin
    • Host: Mouse
    • Label: Biotin
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Mouse Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) (extracellular)-FITC Antibody
    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-FITC Antibody
    A Mouse Monoclonal Antibody to KCa3.1 Channel Conjugated to the Fluorescent Dye FITC
    • Product Code: ALM-051-F
    • Short Description: A Mouse Monoclonal Antibody to KCa3.1 Channel Conjugated to the Fluorescent Dye FITC
    • Host: Mouse
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Mouse Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) (extracellular)-PE Antibody
    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-PE Antibody
    A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Product Code: ALM-051-PE
    • Short Description: A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Host: Mouse
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody
    Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody
    A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR
    • Product Code: AN-170
    • Short Description: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271
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