Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

799 Results
  • Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody
    Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GABBR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGB-001-F
    • Short Description: A Rabbit Polyclonal Antibody to GABBR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
  • Anti-GALR3 (extracellular) Antibody
    Anti-GALR3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Galanin Receptor Type 3
    • Product Code: AGR-013
    • Short Description: A Rabbit Polyclonal Antibody to Galanin Receptor Type 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Galanin receptor type 3, GALNR3, GAL3R
  • Anti-GFR alpha 1 (extracellular) Antibody
    Anti-GFR alpha 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GDNF Family Receptor α1
    • Product Code: ANT-021
    • Short Description: A Rabbit Polyclonal Antibody to GDNF Family Receptor α1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1
  • Anti-GFR alpha 3 (extracellular) Antibody
    Anti-GFR alpha 3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GDNF Family Receptor α3
    • Product Code: ANT-023
    • Short Description: A Rabbit Polyclonal Antibody to GDNF Family Receptor α3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: GFRA3, GFRα3, GDNF family receptor α3, GDNF receptor alpha 3
  • Anti-GFR alpha 4 (extracellular) Antibody
    Anti-GFR alpha 4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GDNF Family Receptor α4
    • Product Code: ANT-024
    • Short Description: A Rabbit Polyclonal Antibody to GDNF Family Receptor α4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: GFRA4, GFRα4, GDNF family receptor α4, GDNF receptor alpha 4, Persephin receptor
  • Anti-GFRAL (extracellular) Antibody
    Anti-GFRAL (extracellular) Antibody
    A Rabbit Polyclonal antibody to GFRAL
    • Product Code: ANT-029
    • Short Description: A Rabbit Polyclonal antibody to GFRAL
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: GDNF Family Receptor Alpha-Like
  • Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody
    KO Validate'
    Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)
    • Product Code: AGR-031
    • Short Description: A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
  • Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
    Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-031-F
    • Short Description: A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
  • Anti-GIPR (extracellular) Antibody
    Anti-GIPR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Product Code: AGR-025
    • Short Description: A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor
  • Anti-GLP1R (extracellular) Antibody
    KO Validate'
    Anti-GLP1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Product Code: AGR-021
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
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