Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Product Code: AFR-014-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR126/ADGRG6 (extracellular) Antibody
    Anti-GPR126/ADGRG6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR126
    • Product Code: AGR-056
    • Short Description: A Rabbit Polyclonal Antibody to GPR126
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR
  • Anti-GPR132 (extracellular) Antibody
    Anti-GPR132 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Product Code: AGR-036
    • Short Description: A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A
  • Anti-GPR142 (extracellular) Antibody
    Anti-GPR142 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR142 (extracellular)
    • Product Code: AGR-082
    • Short Description: A Rabbit Polyclonal antibody to GPR142 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2
  • Anti-GPR142 (extracellular)-FITC Antibody
    Anti-GPR142 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR142 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-082-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR142 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2
  • Anti-GPR151 (extracellular) Antibody
    Anti-GPR151 (extracellular) Antibody
    A rabbit polyclonal antibody to GPR151 (extracellular)
    • Product Code: AGR-081
    • Short Description: A rabbit polyclonal antibody to GPR151 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR151
  • Anti-GPR171 (extracellular) Antibody
    Anti-GPR171 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR171
    • Product Code: AGR-054
    • Short Description: A Rabbit Polyclonal Antibody to GPR171
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GPR171 (extracellular)-PE Antibody
    Anti-GPR171 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: AGR-054-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GPR174 (extracellular) Antibody
    Anti-GPR174 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR174
    • Product Code: AGR-060
    • Short Description: A Rabbit Polyclonal Antibody to GPR174
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79
  • Anti-GPR174 (extracellular)-FITC Antibody
    Anti-GPR174 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR174 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-060-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR174 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79
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