Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

New Products

Product Categories

No categories available

FAQs

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur quis risus id enim vehicula interdum et ac sem.

  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

796 Results
  • Anti-ADAM23 (extracellular) Antibody
    Anti-ADAM23 (extracellular) Antibody
    A Rabbit Polyclonal antibody to ADAM23 (extracellular)
    • Product Code: ANR-155
    • Short Description: A Rabbit Polyclonal antibody to ADAM23 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Disintegrin and Metalloproteinase Domain-Containing Protein 23, MDC3
  • Anti-Adenosine A1 Receptor (extracellular) Antibody
    Anti-Adenosine A1 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Adenosine A1 Receptor (extracellular)
    • Product Code: AAR-009
    • Short Description: A Rabbit Polyclonal antibody to Adenosine A1 Receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Adenosine Receptor A1, Adenosine A1R, ADORA1
  • Anti-Adenosine A1 Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A1 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A1 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-009-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A1 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Adenosine Receptor A1, Adenosine A1R, ADORA1
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular) Antibody
    Anti-Adenosine A2A Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-008
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-PE Antibody
    Anti-Adenosine A2A Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: AAR-008-PE
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular) Antibody
    KO Validate'
    Anti-Adenosine A2B Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Product Code: AAR-003
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: AAR-003-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2b Receptor
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat
    • Applications: Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>3</sub> Receptor (extracellular) Antibody
    Anti-Adenosine A3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Adenosine A3 Receptor (extracellular)
    • Product Code: AAR-035
    • Short Description: A Rabbit Polyclonal antibody to Adenosine A3 Receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ADORA3, A3AR, Adenosine Receptor A3
Load More Results
Loading...

Have questions about our products?

Step-by-Step Protocols