Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-Human FPR1 (extracellular) Antibody
    Anti-Human FPR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR1
    • Product Code: AFR-001
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AFR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human FPR2/ALX (extracellular) Antibody
    Anti-Human FPR2/ALX (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Product Code: AFR-002
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP
  • Anti-Human GLUT3 (extracellular) Antibody
    Anti-Human GLUT3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Product Code: AGT-043
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-Human GLUT3 (extracellular)-FITC Antibody
    Anti-Human GLUT3 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GLUT3 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGT-043-F
    • Short Description: A Rabbit Polyclonal Antibody to GLUT3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-Human GLUT3 (extracellular)-PE Antibody
    Anti-Human GLUT3 (extracellular)-PE Antibody
    A Rabbit Polyclonal antibody to Human GLUT3 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Product Code: AGT-043-PE
    • Short Description: A Rabbit Polyclonal antibody to Human GLUT3 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-Human GPR132/G2A (extracellular) Antibody
    Anti-Human GPR132/G2A (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR132
    • Product Code: AGR-046
    • Short Description: A Rabbit Polyclonal Antibody to GPR132
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G protein-coupled receptor 132, G2 accumulation protein, G2A
  • Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-046-F
    • Short Description: A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: G protein-coupled receptor 132, G2 accumulation protein, G2A
  • Anti-Human GPR43/FFAR2 (extracellular) Antibody
    Anti-Human GPR43/FFAR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-015
    • Short Description: A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-Human GPR55 (extracellular) Antibody
    Anti-Human GPR55 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR55
    • Product Code: ACR-061
    • Short Description: A Rabbit Polyclonal Antibody to GPR55
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: G-protein coupled receptor 55
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