Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-P2Y14 Receptor (extracellular) Antibody
    Anti-P2Y14 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2Y14 Receptor
    • Product Code: APR-018
    • Short Description: A Rabbit Polyclonal Antibody to P2Y14 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor
  • Anti-P2Y2 Receptor (extracellular) Antibody
    Anti-P2Y2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2Y2 Receptor
    • Product Code: APR-102
    • Short Description: A Rabbit Polyclonal Antibody to P2Y2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2Y purinoceptor 2, P2RY2, P2U1
  • Anti-P2Y2 Receptor (extracellular)-FITC Antibody
    Anti-P2Y2 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y2 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-102-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y2 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 2, P2RY2, P2U1
  • Anti-P2Y6 Receptor (extracellular) Antibody
    Anti-P2Y6 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Product Code: APR-106
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-P2Y6 Receptor (extracellular)-APC Antibody
    Anti-P2Y6 Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent Allophycocyanin (APC) fluorophore.
    • Product Code: APR-106-APC
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent Allophycocyanin (APC) fluorophore.
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2RY6
  • Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-106-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-P2Y6 Receptor (extracellular)-PE Antibody
    Anti-P2Y6 Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: APR-106-PE
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-p75 NGF Receptor (extracellular) Antibody
    KO Validate'
    Anti-p75 NGF Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Product Code: ANT-007
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-007-AG
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ANT-007-AO
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
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