Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody
    A Rabbit polyclonal antibody to Prostaglandin E Receptor EP2/PTGER2 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Product Code: APR-064-PE
    • Short Description: A Rabbit polyclonal antibody to Prostaglandin E Receptor EP2/PTGER2 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
  • Anti-PTGFRN (extracellular) Antibody
    A Rabbit polyclonal antibody to PTGFRN (extracellular)
    • Product Code: ANR-212
    • Short Description: A Rabbit polyclonal antibody to PTGFRN (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Prostaglandin F2 Receptor Inhibitor, CD9 Partner 1, CD9P-1, FPRP, Glu-Trp-Ile EWI motif-containing protein F, EWI-F, CD315
  • Anti-PTH1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor
    • Product Code: APR-051
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1
  • Anti-PTH2R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor
    • Product Code: APR-052
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2
  • Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-052-AG
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2
  • Anti-QRFPR/GPR103 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to QRFP Receptor
    • Product Code: AGR-033
    • Short Description: A Rabbit Polyclonal Antibody to QRFP Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27
  • Anti-RAGE (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAGE
    • Product Code: ANR-176
    • Short Description: A Rabbit Polyclonal Antibody to RAGE
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1
  • Anti-RAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP1
    • Product Code: ARR-021
    • Short Description: A Rabbit Polyclonal Antibody to RAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1
  • Anti-RAMP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP2
    • Product Code: ARR-022
    • Short Description: A Rabbit Polyclonal Antibody to RAMP2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
  • Anti-Rat CMKLR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Chemerin Receptor 1
    • Product Code: ACR-033
    • Short Description: A Rabbit Polyclonal Antibody to Chemerin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ
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