Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-SLC3A2 (CD98) (extracellular) Antibody
    Anti-SLC3A2 (CD98) (extracellular) Antibody
    A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Product Code: ANT-192
    • Short Description: A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC
  • Anti-SLC41A2 (extracellular) Antibody
    Anti-SLC41A2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC41A2 Transporter
    • Product Code: ANT-146
    • Short Description: A Rabbit Polyclonal Antibody to SLC41A2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Solute carrier family 41 member 2, Solute carrier family 41 (magnesium transporter), Member 2
  • Anti-SLC4A4 (NBC1) (extracellular) Antibody
    Anti-SLC4A4 (NBC1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NBC1 Transporter
    • Product Code: ANT-075
    • Short Description: A Rabbit Polyclonal Antibody to NBC1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter
  • Anti-SLC6A14 (extracellular) Antibody
    Anti-SLC6A14 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC6A14 Transporter
    • Product Code: AGT-015
    • Short Description: A Rabbit Polyclonal Antibody to SLC6A14 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
  • Anti-SLC7A2 (extracellular) Antibody
    Anti-SLC7A2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2)
    • Product Code: ANT-103
    • Short Description: A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2
  • Anti-SLC7A2 (extracellular)-FITC Antibody
    Anti-SLC7A2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2) Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-103-F
    • Short Description: A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2
  • Anti-Slitrk1 (extracellular) Antibody
    KO Validate'
    Anti-Slitrk1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Slitrk1
    • Product Code: ASR-081
    • Short Description: A Rabbit Polyclonal Antibody to Slitrk1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12
  • Anti-Slitrk2 (extracellular) Antibody
    Anti-Slitrk2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Slitrk2
    • Product Code: ASR-082
    • Short Description: A Rabbit Polyclonal Antibody to Slitrk2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLIT and NTRK-like family member 2, SLITL1
  • Anti-<em>slo</em>β3 (KCNMB3) (extracellular) Antibody
    Anti-sloβ3 (KCNMB3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Product Code: APC-068
    • Short Description: A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3
  • Anti-Somatostatin Receptor Type 1 (extracellular) Antibody
    Anti-Somatostatin Receptor Type 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Somatostatin Receptor Type 1
    • Product Code: ASR-001
    • Short Description: A Rabbit Polyclonal Antibody to Somatostatin Receptor Type 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SSTR1, SS1R, SRIF-2, SRIF2A
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