Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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799 Results
  • Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody
    Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-063-AO
    • Short Description: A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Stromal interaction molecule 1, GOK
  • Anti-Stomatin-like Protein 1 (extracellular) Antibody
    Anti-Stomatin-like Protein 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Stomatin-Like Protein 1
    • Product Code: AIP-001
    • Short Description: A Rabbit Polyclonal Antibody to Stomatin-Like Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: STOML1, SLP-1, STORP, Slipin-1, hUNC-24
  • Anti-SVCT2/SLC23A2 (extracellular) Antibody
    Anti-SVCT2/SLC23A2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SVCT2 Transporter
    • Product Code: AST-022
    • Short Description: A Rabbit Polyclonal Antibody to SVCT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2
  • Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody
    Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Taste Receptor Type 1 Member 3
    • Product Code: ATR-016
    • Short Description: A Rabbit Polyclonal Antibody to Taste Receptor Type 1 Member 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Sweet Taste Receptor T1R3, T1R3
  • Anti-Tetraspanin 18 (extracellular) Antibody
    Anti-Tetraspanin 18 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin 18 (extracellular)
    • Product Code: ANR-189
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin 18 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN18, Tetraspanin-18
  • Anti-Tetraspanin 2 (extracellular) Antibody
    Anti-Tetraspanin 2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)
    • Product Code: ANR-188
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetraspan NET-3, TSPAN2
  • Anti-Tetraspanin 7 (extracellular) Antibody
    Anti-Tetraspanin 7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)
    • Product Code: ANR-187
    • Short Description: A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.
  • Anti-Tetraspanin-3 (extracellular) Antibody
    Anti-Tetraspanin-3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)
    • Product Code: ANR-185
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8
  • Anti-Thromboxane A2 Receptor (extracellular) Antibody
    Anti-Thromboxane A2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Thromboxane A2 Receptor (extracellular).
    • Product Code: APR-095
    • Short Description: A Rabbit Polyclonal antibody to Thromboxane A2 Receptor (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Prostanoid TP receptor, TXA2-R, TBXA2R
  • Anti-Thromboxane A2 Receptor (extracellular)-FITC Antibody
    Anti-Thromboxane A2 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-095-F
    • Short Description: A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostanoid TP receptor, TXA2-R, TBXA2R
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