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α-Conotoxin GI

α-Conotoxin G1

A Potent Antagonist of α1/β1/δ/γ nAChR

Cat #: STC-500
Alternative Name α-Conotoxin G1
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 1438 Da
    Purity: >98% (HPLC)
    Effective concentration 10 nM - 1 μM.
    Sequence ECCNPACGRHYSC.
    Modifications Disulfide bonds between Cys2-Cys7, Cys3-Cys13. Cys13 - Cysteine amide.
    Structure
    Molecular formula C55H80N20O18S4.
    CAS No.: 76862-65-2
    Activity α-Conotoxin GI inhibits α/δ site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI1,2.
    References-Activity
    1. Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
    2. Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs α-Conotoxin GI inhibits the muscle (α1/β1/δ/γ) nAChR channels heterologously expressed in Xenopus oocytes.
      Alomone Labs α-Conotoxin GI inhibits the muscle (α1/β1/δ/γ) nAChR channels heterologously expressed in Xenopus oocytes.
      A. Time course of α-Conotoxin GI (#STC-500) action on α1/β1/δ/γ nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at –60 mV and oocytes were stimulated by exposure to 1 mM acetylcholine every 100 seconds. 100 nM α-Conotoxin GI were perfused during the period marked by the bar. B. Superimposed traces of α1/β1/δ/γ nAChR channel current in the absence and presence of 100 nM α-Conotoxin GI (taken from the experiment in A).
    References - Scientific background
    1. Gray, W.R. et al. (1981) The J. Biol. Chem. 256, 4734.
    2. Nishiuchi, Y. and Sakakibara, S. (1982) FEBS LETT. 148, 260.
    3. Guddat, L.W. et al. (1996) Biochemistry 35, 11329.
    4. Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
    5. Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
    Scientific background

    α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR).

    α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.

    Target α1/β1/δ/γ nAChR
    Peptide Content: 100%
    Lyophilized Powder
    Last update: 01/09/2021

    α-Conotoxin GI (#STC-500) is a highly pure, synthetic, and biologically active peptide toxin.

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    For research purposes only, not for human use

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