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1-Norokadaone

An Inactive Homologue of Okadaic Acid and Okadaol, Inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A

Cat #: N-750
Lyophilized Powder yes
  • Bioassay Tested
    • Source Synthetic
    MW: 758.99
    Purity: >98%
    Structure
    Chemical name 1-[11-Hydroxy-2-[(E)-4-[4-hydroxy-2-[1-hydroxy-3-(3- methyl-1,7-dioxaspiro[5.5]undecan-2-yl)butyl]-3-methylidenespiro [4a,7,8,8a-tetrahydro-4H-pyrano[3,2-b]pyran-6,5'-oxolane]-2'-yl]but-3-en-2-yl]-4-methyl-1,7-dioxaspiro[5.5]undec-4-en-8-yl]propan-2-one.
    Molecular formula C43H66O11.
    CAS No.: 131204-29-0
    Activity 1-Norokadaone is an inactive analogue of okadaic acid (a PP1 and PP2A inhibitor) even at high concentrations1. It may be used as a negative control vis-à-vis okadaic acid.
    References-Activity
    1. Gurland, G. and Gundersen, G.G. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 8827.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO, ethanol, acetone or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs 1-Norokadaone serves as a negative control for Okadaic Acid in modulating the phophatase activity of PP-2A.
      Alomone Labs 1-Norokadaone serves as a negative control for Okadaic Acid in modulating the phophatase activity of PP-2A.
      Cells were grown to 70% confluence and serum starved for 18 h. For in vivo phosphatase activity, the growth medium was replaced with HENK’s and the cells were preincubated in the presence or absence of 100 nM Okadaic Acid (ammonium salt) (#O-800), (or Okadaic Acid (sodium salt) (#O-900)) and 100 nM 1-Norokadaone (#N-750) followed by a 20 min incubation with or without 20 µM NEM. The cells were then stimulated with 5 ng/ml PDGF for 30 min. At the end of the incubation, cell proteins were resolved by SDS-PAGE and probed with anti-phospho-Akt antibodies.
    References - Scientific background
    1. Bastan, R. et al. (2001) Eur J Pharmacol, 430, 135.
    2. Honkanen, R.E. et al. (1994) Toxicon 32, 339.
    3. Nishiwaki, S. et al. (1990) Carcinogenesis 11, 1837.
    Scientific background 1-Norokadaone is structural analogue of Okadaic acid. This compound is a less active homologue than the Okadaic Acid and Okadaol as inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A, and is thus widely used as negative control for Okadaic Acid1-3.
    Lyophilized Powder
    Last update: 13/10/2022

    1-Norokadaone (#N-750) is a highly pure, synthetic, and biologically active compound.

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    For research purposes only, not for human use

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