ADAM22 (extracellular) Blocking Peptide (#BLP-NR120) is the original antigen used for immunization during Anti-ADAM22 (extracellular) Antibody (#ANR-120) generation. The blocking peptide binds and ‘blocks’ Anti-ADAM22 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of ADAM22 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-ADAM22 (extracellular) Antibody (#ANR-120), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. ADAM22 staining (green) appears in Purkinje cells (vertical arrows) and in dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei are stained with DAPI (blue).
- (C)HNDDAKTGITLSGNG, corresponding to amino acid residues 715-729 of mouse ADAM22 (Accession Q9R1V6).