AKAP12 Blocking Peptide (#BLP-PZ033) is the original antigen used for immunization during Anti-AKAP12 Antibody (#APZ-033) generation. The blocking peptide binds and ‘blocks’ Anti-AKAP12 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of AKAP12 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AKAP12 Antibody (#APZ-033), (1:400), followed by goat anti-rabbit-AlexaFluor-488. AKAP12 immunoreactivity (green) is detected in Purkinje cells and their dendritic trees (arrows). Cell nuclei are stained with DAPI (blue).
- (C)GNLTKESPDTNGPK, corresponding to amino acid residues 1639-1652 of mouse AKAP12 (Accession Q9WTQ5).