Overview
Type: Synthetic peptide
Form: Lyophilized powder
Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide (#BLP-AR012) is the original antigen used for immunization during Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012) generation. The blocking peptide binds and ‘blocks’ Anti-Angiotensin II Receptor Type-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse MS1 endothelial cells:1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, (AAR-012), (1:400).
2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide (#BLP-AR012).
Western blot analysis of rat brain membranes:1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (AAR-012), (1:500).
2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide (#BLP-AR012).
Expression of Angiotensin II receptor type-2 in rat brainImmunohistochemical staining of rat brain sections using Anti-Angiotensin Receptor Type-2 (extracellular) Antibody (#AAR-012). A. AT2 receptor expressing neurons (red) are scattered in the paraventricular nucleus of the hypothalamus (arrows), in the vicinity of the 3rd ventricle (3rd V). B. AT2 receptor expressing neurons in the supraoptic nucleus (arrows), adjacent to the optic nerve. Nissl is used as the counterstain (blue).
Properties
Sequence
- DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351).
Accession (Uniprot) Number P35351
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
