Angiotensin III

Ang III, Angiotensin-3, des-As1-angiotensin II
A Ligand of AT1 and AT2 G-Protein Coupled Receptors
    Cat #: GPA-130
    Alternative Name Ang III, Angiotensin-3, des-As1-angiotensin II
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 931.1 Da.
    Purity: >98% (HPLC)
    Effective concentration 1 nM - 1 μM.
    Sequence RVYIHPF.
    Structure
    • Angiotensin III
    Molecular formula C46H66N12O9.
    CAS No.: 13602-53-4.
    Activity Angiotensin III is an endogenous ligand of the AT1 and AT2 receptors and like Angiotensin II, regulates blood vessel tonus, blood pressure, and salt and water balance1-2.
    References-Activity
    1. Devynck, M. A. et al. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 4092.
    2. Reaux, A. et al. (2001) Trends Endocrinol. Metabol. 12, 157.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Our bioassay
    • Angiotensin III
      Alomone Labs Angiotensin III induces MAPK activation in IEC-6 cells similar to Angiotensin II.
      Cells were incubated in serum depleted medium for 2 h and then stimulated with 0.1 µM and 1 µM Angiotensin III (#GPA-130) for 10 min or with 0.1 µM and 1 µM Angiotensin II (#GPA-100) for comparison. Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.
    References - Scientific background
    1. Wright, JW. et al. (2011) Prog. Neurobiol. 95, 49.
    2. Blair-West, JR. et al. (1980) J. Endocrinol. 87, 409.
    3. Fei, DT. et al. (1980) Life Sci. 27, 1495.
    4. Fink, GD. et al. (1985) Am. J. Physiol. Endocrinol. Metab. 249, E201.
    5. Jensen, LL. et al. (1989) Brain Res. 490, 48.
    6. Wright, JW. et al. (1985) Am. J. Physiol.Regulatory Integrative Comp. Physiol. 249, R514.
    7. Wright, JW. et al. (1989) Am. J. Physiol.Regulatory Integrative Comp. Physiol. 257, R1551.
    Scientific background Angiotensin III (Ang III) is a hexapeptide formed from cleavage at the N-terminus of Angiotensin II (Ang II)1, a key factor in the Renin-Angiotensin-Aldosterone (RAAS) system. In peripheral Ang systems, Ang II is the main effector peptide in the systemic circulation, although exogenous Ang III can be as potent as Ang II in, for example, stimulating aldosterone secretion2 or inhibiting renin release3. In the rat brain, Ang III was found to be equipotent with Ang II as a pressor agent or dipsogen4-7 and was bound as avidly to the nervous system as Ang II. Angiotensin receptor subtype AT1 has greater affinity towards Ang II and is also responsive to Ang III, while the AT2 receptor subtype appears to be more sensitive to Ang III but less responsive to Ang II1.
    Target Angiotensin II receptors
    Net Peptide Content: 100%
    Last update: 24/01/2020

    Angiotensin III (#GPA-130) is a highly pure, synthetic, and biologically active peptide.

    For research purposes only, not for human use
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